Practical application of cytochrome P450.
نویسنده
چکیده
Recent progress on the application of cytochrome P450 (P450) to bioconversion processes, biosensors, and bioremediation were reviewed. Because regio- and enantioselective hydroxylation makes chemical synthesis difficult, a bioconversion process using P450 would be quite attractive. One of the most successful industrial applications of P450 may be the bioconversion process for pravastatin formation using a Streptomyces carbophilus CYP105A3. Unfortunately, practical application of P450s in the bioconversion process is limited because of their low stability, low activity and co-factor dependency. However, directed evolution is expected to generate useful P450 biocatalysts for a wide range of substrates. Shunt pathways of CYP152A1, CYP152A2, and CYP152B1 are notably promising for practical application, because these P450s require neither reduced nicotinamide adenine dinucleotide phosphate (NAD(P)H) nor electron donor proteins, and efficiently catalyze using hydrogen peroxide. A P450 biosensor using biochip technology is expected to become a tool for rapidly determining drugs and endogenous substances in plasma at a low cost. Bioremediation of dioxins and polychlorinated biphenyls (PCBs) by the CYP1 family appears to be possible by using suicidal, genetically engineered microorganisms. The P450 superfamily has tremendous potential for practical applications in various fields.
منابع مشابه
Cloning and gene expression of cytochrome P450 gene from Alcanivorax borkumensis Bacterium
Alcanivorax borkumensis is a marine bacterium that has ability to grow on limited substrates that mainly is alkanes. The ability to use wide range of hydrocarbons is advantage of this bacterium to other marine community bacteria. A. borkumensis have two genetic systems for alkane biodegradation. The First system is alkane hydroxylase (alk-B1and alk-B2) and the second system is...
متن کاملCloning and gene expression of cytochrome P450 gene from Alcanivorax borkumensis Bacterium
Alcanivorax borkumensis is a marine bacterium that has ability to grow on limited substrates that mainly is alkanes. The ability to use wide range of hydrocarbons is advantage of this bacterium to other marine community bacteria. A. borkumensis have two genetic systems for alkane biodegradation. The First system is alkane hydroxylase (alk-B1and alk-B2) and the second system is...
متن کاملExpression of cytochrome P450 and glutathione S-transferase in human bone marrow mesenchymal stem cells
Currently several studies are being carried out on various properties of mesenchymal stem cells (MSCs)however there are a few investigations about drug metabolizing properties of these cells. The aim of thisstudy was to measure the key factors involved in drug metabolism in human bone marrow MSCs. For thispurpose, cellular glutathione (GSH), glutathione Stransferase (GSTs) and...
متن کاملEvaluation of lentinan effects on cytochrome P450 activity in rats by a cocktail method
Objective(s): In this study, a cocktail of probe drugs was used to assess whether lentinan could influence the activities of rat enzymes CYP3A4, CYP2D6, CYP1A2, CYP2C19, and CYP2C9 in vivo. Materials and Methods: Fourteen days after intraperitoneal injection of lentinan, rats were given an oral dose of a cocktail solution containing phenacetin, tolbutamide, omeprazole, metoprolol, and midazolam...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Biological & pharmaceutical bulletin
دوره 35 6 شماره
صفحات -
تاریخ انتشار 2012